Handling compressed gas cylinders and changing regulators can be intimidating. However, there are steps you can take to feel at ease and people you can lean on to provide hands-on training. Please review Sarah Arrowsmith's PowerPoint for details.
Juan Maldonado Ortiz, Manager of the SCEB Microbiome Analysis Lab, has identified this one minute video, which us about sharps and safety in the lab and was created by the EH&S Biosafety Team. Please watch it as it will keep you and others safe at work.
Introduction and Scope:
The Center for Environmental Biotechnology is a group comprised of nearly 80 people, including several faculty members and their research labs. As a result of the large number of personnel and multitude of ongoing research projects, there is a very large amount of supplies being ordered. Often, several individuals will order the same items. This policy it intended to reduce repetitious ordering of common supplies, make sure that only allowable supplies are charged to sponsored accounts, and to track the deliveries and ensure that they are given to the correct people.
Policy for placing orders:
All order requests begin with an email request to the Center Lab Manager including the following information:
This information ensures that we have documentation for who, what and why we place orders, as required by account auditors.
Too often, the regulators for many of our gas tanks have been so over-tightened that it takes using a hammer on the wrench in order to loosen them. Tightening regulators this much causes damage to the threads of the regulator and the gas cylinder. Do not over-tighten the regulator!
It’s likely that over-tightened regulators happen because users experience false leaks using the Snoop (surfactant) test.
Here is guidance for using Snoop properly:
Here is a useful YouTube demonstration using liquid Snoop for finding gas leaks.
Here is a great video from Harris that describes how to change a gas cylinder.
But it is a ‘dry heat’ is the statement we so often hear from friends, family and the meteorologists on the local news broadcasts during the summer months in the Tempe area. So often that we frequently tend to ignore how serious these warnings actually are. In Phoenix this time of year we frequently have what the weather service term “Excessive Heat” warnings and advisories.
Heat Advisories are not just based on the recorded ambient temperature but on what is called the Heat Index – this Heat Index is based on a formula using the temperature and the relative humidity. Heat Advisories are issued when the Heat Index is at 105 °F (40.6°C) for 3 consecutive hours during a day OR if the temperature is to exceed 115°F (46°C) for any length of time during a day. These civic alerts are issued to alert the citizens that the local weather conditions are dangerous. These excessive Heat Index readings for the Tempe area can be quite dangerous for small children, the elderly and individuals with chronic illnesses such as Hypertension, Cardiovascular disease and diabetes as well as for healthy active individuals. Just in the Phoenix area alone there, are on average, 2000 people treated every year in emergency rooms for Heat-Related Illnesses and on average 125 people die in Arizona from the heat.
There are three levels of heat-related illness to be aware of.
There are several common sense tips to help with preventing Heat-Related illnesses which include
With a little common sense and taking reasonable precautions the risk of Heat-Related Illness can be reduced even in Phoenix with our “dry heat” extremes.
Introduction and Scope:
The Center for Environmental Biotechnology is a group comprised of nearly 75 people, and several Faculty members. As a result of the large number of personnel and their ongoing research projects, there is a very large amount of supplies being ordered – many times several individuals will be ordering the same items. This policy it intended to track the deliveries and attempt to ensure that the right items are given to the correct persons.
Policy for Deliveries:
The Center Laboratory Manager will accept all deliveries for the Center. The Packing slips will be matched up with the ordering paperwork. A note tag will be attached to the item(s) with the recipient’s name and the item will be placed in the appropriate location to be collected by the person to which it belongs. Smaller items will be place on the shelf just inside A207, large items will be placed on the desk at this location, items requiring refrigeration will be place on the top shelf in the refrigerator just inside the lab at A207, items requiring -20 freezer storage will be placed in the Freezer portion of this refrigerator and items requiring -80 storage will be placed in the freezer #61 located in the 2nd floor lab refrigerator corridor. An Email will be sent to the person who placed the order (or their designated person) to notify them of the delivery. No item is to be removed from the delivery area if it has not been tagged with the ordering person’s name.
In the absence of the Center Laboratory Manager deliveries will be handled in the following manner:
1. A small list of center members are designated as able to accept deliveries.
2. The person accepting deliveries will sign each of the packing slips.
3. This person accepting the deliveries will put all the packing slips on the Center Laboratory Managers desk for further processing
4. This person receiving will determine if the delivery item needs refrigerated or freezer storage and put the item in the proper temperature storage.
5. If the identification of the ordering person can be determined from the packing slip then the individual accepting deliveries is to put a tag on the item with this name. If there is no indication as to who placed the order request then no tag is to be attached.
6. No item is to be removed from the delivery area if it has not been tagged with the ordering person’s name. Only the ordering person may collect the delivered items.
The following guideline for the use, storage and transportation of compressed gas cylinders has been prepared for use by the faculty, staff, students and other individuals who may need to use compressed gas cylinders in the Center for Environmental Biotechnology. This policy is in accordance with applicable codes, standards, ASU Fire Prevention and Safety Plans, and University Policies and Procedures. Those individual researchers involved in the use of the compressed gas cylinder are ultimately responsible for proper storage, use and transportation of these tanks. The Center Director, faculty and staff are also responsible for providing instruction, a safe environment and direction for the students, visiting scholars and others who may need to use these tanks in the EB Center.
Empty Tank Removal:
Gas Tank Training requirements:
ASU's Compressed Gases Safety Program detail may be found here.
All members of the Center for Environmental Biotechnology must complete each calendar year the following safety training sessions:
Each of these classes is available on line through the My-ASU Blackboard system (click on the 'Courses' tab and search for courses).
The training must be completed no later than the end of each January to be compliant and retain access to the Biodesign Institute and EB Labs. This renewal of all these training classes is required – no matter how recently the previous training was done. Upon completion of the on-line session, the researcher must email confirmation of completion to the Center Lab Manager or print out the verification of completion for each session and submit this to the Center Laboratory Manager for documentation.
1. Additionally: anyone using Radioactive Material must attend an annual Radiation Training Refresher class – this will be scheduled by the Center Laboratory Manager to accommodate the most people in one session. Those individuals not able to attend the scheduled group session will be required to arrange for this training on their own, bringing the documentation of completion to the Center Laboratory Manager.
2. Additionally: any one working with Humans Subjects samples must complete the NIH Human Subjects Training, available on-line and is valid for 36 months. The Center Laboratory Manager will inform all researchers when they need to take this training, which is available on line.
3. Additionally: anyone needing to drive a rental vehicle while on ASU business must have completed Defensive Driving Training, which is available on line and is valid for 4 years.
4. Additionally: all Environmental Biotechnology Center members needing to drive a golf cart on campus completion of Driving on the Mall is required
Scope, Application and Purpose:
The Purpose of this Swette Center for Environmental Biotechnology Policy for the Importation, Transportation, Storage and Destruction of Soil and Water Samples is to ensure that the Center will follow all the regulations for the handling of soil and water samples as directed by the United States Department of Agriculture, Animal and Plant Health Inspection Service (APHIS).
The Permit is held by Lab Manager Diane Hagner for the Swette Center for Environmental Biotechnology and she is responsible for compliance with all regulations. The permit allows the Center to obtain soil samples in the quantity of 3 pounds or less, which have not been previously sterilized or pre-treated, as that process would destroy the sample value for the purpose of our research. Samples will not be received from any locations or countries currently under sanction by the US Government.
Procedure for Sending Soil Samples to our Lab:
All collaborators are required to notify the Swette Center for Environmental Biotechnology a minimum of 30 days prior to requesting to ship any samples to our lab. The PPQ Form 550 Black-White label will be completed by our Staff and sent to them to attach to the exterior of the shipping carton as well a copy of the current permit to be included inside with the documents describing the sample. The sample material must be free from any plant or animal material; the sample must only be soil.
The soil samples must be shipped in a securely closed, water-tight/leak-proof primary container which must be then contained in a second, durable, watertight/leak-proof shipping container. This double packing is required to ensure that the soil sample cannot be introduced into the environment in the event of the package being damaged in transit. Samples may not be hand-carried under our permit but must be shipped by approved 3rd party carriers. Hand-carrying of soils requires a special request and amendment to our permit (see below for the process). The exterior of the packing containers and the secondary shipping container must be free from any contamination from the soil sample.
Soil samples will be retrieved form the shipping dock within 2 hours of arrival and moved to the lab for storage. Transferring of the regulated soil away from SCEB labs requires that any recipient of the soil also have a US Government permit to receive soil samples. The shipment to that facility will be under their permit to receive soil samples – our permit is just to receive samples.
Procedure for Hand-Carrying of Soil Samples to our Lab:
A written request for permission to transport soil must be sent to the Lab Manager (the APHIS Permit Holder for the SCEB lab) a minimum of 30 days prior to the time that soil samples are to be hand-carried either into the United States from foreign countries or from another State within the United States.
Requests for permission to hand-carry soil samples may take 3-6 months to be approved.
This request must include:
Once permission to transport soil is given, the sample must be contained in a closed, leak-proof container which is placed inside a second container' both of which are clearly marked as to the contents and have the proper stickers from APHIS clearly visible. If the soil sample is transported by airlines it is NOT permitted to travel as a checked item but must travel as carry-on luggage, and so must meet the size requirements for carry-on packages for the airline. The soil samples must be less than 3 pounds.
The authorization to hand carry samples may not be transferred to another individual – if the person designated to carry the sample has a change of plans or schedule the Lab Manager must be notified immediately and the permit is cancelled – the soil cannot be transported.
Procedure for Storage and Use of Soil Samples:
All soil samples must be clearly marked as to place of origin and researcher scheduled to be using the samples. All samples are to be logged into the Soil Samples Use Log book, maintained at the Lab Manager’s desk, which will also be a record of the location in the SCEB lab that the soil samples are stored. All aliquots of soil samples must be recorded in the Soil Samples log book as they are taken for lab use. The log book must contain the following information for each sample:
Procedure for Destruction of Soil Samples:
The samples will be autoclaved - using one of the following Dry Heat treatments; temperature determined when the sample reaches the min. temp, using a temperature probe for verification:
Transfer of Soil Samples to Other Facilities from ASU:
Transfer of any soil samples from the Swette Center for Environmental Biotechnology will require that the facility requesting the soil is also in possession of an APHIS permit from the USDA and that an MTA has been approved by ASU – ORIA. We are not permitted to send soil to a 3rd party location unless they have the proper permits. A PPQ 525 permit to transfer soil samples must be obtained by our lab.
All researchers using soil samples in their research must successfully complete initial soil sample training prior to being allowed to use soil samples in the SCEB lab (regardless of their previous experience) and then repeat the training every two years.
Procedures for Sending Water Samples to our Lab:
All water samples must be cleared for use 30 days prior to sending them to SCEB for use in the lab.
We must submit the declaration and the experimental plan to ASU EHS for clearance prior to the water being sent to the lab. Once clearance is given, all transportation of these water samples must be via a 3rd party shipper.
When approved samples are no longer needed they will be autoclaved and discarded.
Samples sent not following the above procedures will be impounded by the Laboratory Manager, reported to ASU EHS and ORIA who will determine the outcome of these samples which are in violation of governmental policies, which may result in the samples being required to be autoclaved and discarded without use.